General Resources
This page offers resources providing a good introduction into the cryoEM/ET world. Please be patient, as this page is a work in progress!
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Getting Started in Cryo-EMCaltech course delivered by Grant Jensen on the fundamentals of cryoEM/ET with coverage on the anatomy of the electron microscope, Fourier transforms and more
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CryoEM 101Training material developed by labs at the University of Utah, providing the workflow overview for both CryoEM and CryoET
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MRC Laboratory of Molecular Biology (LMB) Cryo-EM LecturesRecordings from the 2017 Cryo-EM course
MYSCOPE Microscopy TrainingDeveloped by Microscopy Australia to provide an educational platform to learn about the basics of microscopy and simulate operation of various microscopy systems such as FIB, SEM, etc.
Cryo-EM PrinciplesSeries of lecture videos from Yale University on the principles underlying 3D reconstruction from raw 2D images
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Workflow Technique Tutorials
As depicted in the figure above, the cryoET workflow involves the following steps:
- Sample Preparation
- Vitrification
- Identification and Localization of ROIs by cryo-CLEM
- Data Collection
- Data Processing
We have developed optimized lab protocols for each of these steps in this structure determination pipeline. Click on the protocol to be directed to a PDF file.
Module I: Culturing Cells on GridComing soon...
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Module II: VitrificationThis protocol provides a step-by-step walkthrough on how to vitrify biological specimens on a Leica EM GP system.
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Module III: Identification and Localization of ROIs by cryo-CLEMThis protocol guides you through how to use cryo-CLEM to locate target molecules for subsequent cryoET imaging.
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Module IV: Thinning by Cryo-FIBComing soon...
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Module V: Data CollectionComing soon...
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Module VI: Tilt Series Alignment and Reconstruction by IMODComing soon...
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Module VII: EMAN2 Data ProcessingEMAN also has a wiki page with well-written tutorials and documentation for the software. Questions can be submitted to the developers the EMAN2 Google Group.
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Relevant Webinars/Workshops/Conferences
- SBGrid Consortium - recorded webinars on various software applications used in structural biology
- Simons Electron Microscopy Center (SEMC) - offers workshops on single particle analysis, 3D reconstruction, operation of Krios, and more
- Microscopy & Microanalysis (M&M)
- American Crystallographic Association (ACA): The Structural Science Society
- 3DEM Meetings and Workshops
National Network for CryoET
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The National Network for CryoET is comprised of:
For more information about the network and submitting user proposals, visit: https://www.cryoetportal.org/. |